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Real-time Cytotoxicity Assays in Human Whole Blood
Published on: November 7, 2014
在不确定的献血者中,T细胞反应和先前接触C型肝炎病毒的情况
Nasser Semmo1, Eleanor Barnes, Craig Taylor
1Peter Medawar Building for Pathogen Research, University of Oxford, Oxford, UK.
Lancet (London, England)
|January 25, 2005
概括
许多血液捐献者具有不确定的C型肝炎病毒 (HCV) 检测结果显示T细胞反应,表明先前的短暂HCV暴露. 这一发现有助于理解不确定的捐赠和潜在的过去感染.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 输血医学 输血医学
背景情况:
- 常规的献血者查可以确定患有型肝炎病毒 (HCV) 感染的个体.
- 一些捐赠者表现出不确定的结果,其特点是抗体反应较弱或受限,但通常是HCV RNA负的.
- 这些不确定的结果的原因,特别是在没有可检测的病毒血症的情况下,仍然不清楚.
研究的目的:
- 调查假设,过时的,过去接触C型肝炎病毒可能解释不确定的献血结果.
- 为了评估血液供献者中病毒特异性T细胞反应的存在,不确定查结果.
主要方法:
- 对T细胞反应的ex-vivo分析是在不确定的捐赠者的血液样本上进行的.
- 在不确定的献血者和对照组之间对T细胞反应进行了比较.
- 专注于识别针对型肝炎病毒核心衍生的T细胞反应.
主要成果:
- 在30名测试的不确定的献血者中,在15名中检测到病毒特异性T细胞反应.
- 在对照组中没有观察到病毒特异性T细胞反应 (p=0.0013).
- 确定的T细胞反应主要针对HCV的核心衍生.
结论:
- 特定的T细胞反应的存在强烈地表明,在不确定的献血者中,很大一部分人以前曾接触过C型肝炎病毒.
- 这些发现表明,不确定的结果可能反映出已解决或过去过渡的HCV感染,而不是活跃的感染.
- 这种理解可以完善捐赠者查协议,并改善输血医学中HCV检测结果的解释.
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