通过cadherin细胞质域调节胚胎细胞粘附
1Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, San Diego, California 92186.
Cell
|April 17, 1992
概括
卡德林通过与卡特宁的细胞质相互作用来调节胚胎细胞粘附. 一种突变的N-cadherin缺乏其细胞外域抑制了细胞粘附,揭示了关键的调节区域.
科学领域:
- 发展生物学 发展生物学
- 细胞粘附 细胞粘附
- 分子生物学分子生物学
背景情况:
- 不同的细胞粘附对于胚胎发育至关重要.
- 卡德林是关键的细胞粘附分子,与细胞内氨酸相互作用.
- 细胞质相互作用在卡德林功能的确切作用尚未完全阐明.
研究的目的:
- 研究N-cadherin细胞质域在胚胎发育期间调节细胞粘附中的作用.
- 为了确定N-cadherin细胞质域的特定区域是否可以抑制细胞粘附.
- 为了检查一个截断的N-cadherin突变对catenin与其他cadherins结合的影响.
主要方法:
- 产生一个Xenopus N-cadherin突变体,缺乏细胞外域.
- 在Xenopus胚胎中突变N-cadherin的表达.
- 对结果对胚胎细胞粘附和猫内因结合的影响进行分析.
主要成果:
- N-cadherin突变体的表达显著抑制了胚胎细胞粘附.
- 在N-cadherin细胞质域内至少有两个不同的区域被确定为抑制粘附.
- 突变的cadherin被证明可以干扰catenins与E-cadherin的结合.
结论:
- 卡德林介导的细胞粘附是通过涉及卡德林细胞质域和细胞内氨酸的相互作用来调节的.
- 这些细胞质相互作用可以调节卡德林功能,可能在形态发生过程中发挥作用.
- 这些发现突出了组织发育过程中调节细胞粘附的机制,特别是当多种类型的干林被共同表达时.
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