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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Published on: April 6, 2012
微阵列分析表明,一些microRNAs降低了大量的目标mRNAs的下调
Lee P Lim1, Nelson C Lau, Philip Garrett-Engele
1Rosetta Inpharmatics, Merck and Co, 401 Terry Avenue N, Seattle, Washington 98109, USA. lee_lim@merck.com
Nature
|February 3, 2005
概括
微RNAs (miRNAs) 调节基因表达. 将特定的miRNA引入人体细胞改变了基因表达特征,并降低了许多向信使RNA的调节,揭示了它们更广泛的调节作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 微RNAs (miRNAs) 是小型的非编码RNAs.
- 它们在物种间的转录后基因调节中起着至关重要的作用.
- 了解miRNA功能是解读基因表达控制的关键.
研究的目的:
- 为了研究特定的microRNAs对细胞转录水平的影响.
- 为了确定miRNA是否可以改变人类细胞中的全球基因表达特征.
- 确定微RNA及其调节机制的直接目标.
主要方法:
- 特定的miRNAs (miR-124和miR-1) 转移到人类细胞中.
- 利用微阵列来分析信使RNA配置文件的变化.
- 对miRNA-目标相互作用的生物信息分析,重点关注3'未翻译区域.
主要成果:
- 传染miR-124诱导了类似大脑的表达特征,而miR-1诱导了类似肌肉的特征.
- 对于每个miRNA,大约100个信使RNA点在传染后12小时内下调.
- 观察到目标信使RNA 3'未翻译区域与miRNA 5'区域结合的显著倾向.
结论:
- 甲基动物的miRNAs可以显著降低多个目标转录的水平.
- 组织特异性的miRNAs,如miR-1和miR-124,对比以前理解的更多的目标进行下调.
- 这些发现突出了miRNAs在建立和维持组织特异性基因表达模式中的关键作用.
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