CD36是一种二甲基糖的传感器
Kasper Hoebe1, Philippe Georgel, Sophie Rutschmann
1Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA.
Nature
|February 4, 2005
概括
CD36是某些微生物分子的关键传感器,由托尔类受体2 (TLR2) 识别. 这一发现揭示了CD36的存在.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 分子生物学分子生物学
背景情况:
- 收费类受体2 (TLR2) 识别了各种微生物组成部分,可能涉及适应蛋白.
- 确切的机制和TLR2配体识别的范围仍然不完全理解.
研究的目的:
- 研究CD36在TLR2介导免疫反应中的作用.
- 为了识别由TLR2识别的特定微生物配体,这些配体依赖CD36.
主要方法:
- 使用Cd36 (Cd36(obl)) 中具有N-乙烯-N-酸尿素诱导的无意义突变的小鼠模型.
- 评估巨细胞对各种TLR2连接体的敏感性,包括细菌脂和脂铁醇酸.
- 评估同卵性Cd36(obl) 鼠对金黄色葡萄球菌感染的易感性.
主要成果:
- Cd36(obl) 巨细胞对特定的TLR2配体,如MALP-2的R-反体和脂铁醇酸,表现出不敏感.
- 同卵性Cd36(obl) 鼠对金黄色葡萄球菌感染的敏感性增加.
- Cd36(obl) 巨细胞仍然可以检测到其他TLR2联体,表明有选择性的CD36依赖.
结论:
- CD36作为一种选择性和非冗余的传感器,用于通过TLR2/6异构体发送信号的微生物二甲基糖化物.
- CD36通过感知特定的TLR2连接体,在对某些细菌和真菌病原体的天生的免疫力中发挥关键作用.
- 这一发现扩大了CD36已知的功能,超越了内源分子识别,包括微生物传感.
相关概念视频
01:27Transducer Mechanism: Enzyme-Linked Receptors
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
01:28Insulin: The Receptor and Signaling Pathways
Insulin action is mediated through a receptor tyrosine kinase, akin to the IGF-1 receptor. The number of receptors per cell varies significantly, from 40 on erythrocytes to 300,000 on adipocytes and hepatocytes. The insulin receptor consists of linked α/β subunit dimers, forming a heterotetramer glycoprotein with two extracellular α subunits and two β subunits spanning the membrane. The α subunits inhibit the inherent tyrosine kinase activity of the β subunits, but this inhibition is released...
01:17Microbial Biosensors
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...