产后的l1+心脏细胞进入完全差异化的心肌细胞系
Karl-Ludwig Laugwitz1, Alessandra Moretti, Jason Lam
1Institute of Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla, California 92093, USA.
Nature
|February 11, 2005
概括
研究人员在产后心脏中确定了原生心脏原生细胞 (心脏细胞). 这些isl1+细胞可以更新和分化为心肌细胞,提供有关心脏发育和疾病的见解.
科学领域:
- 心血管生物学 心血管生物学
- 发育生物学 发展生物学
- 干细胞生物学 干细胞生物学
背景情况:
- 在产后心脏中发现本地心脏前体细胞的证据有限.
- 了解心脏原生细胞生物学对于心脏病研究至关重要.
- 心脏细胞谱系形成机制仍然不完全理解.
研究的目的:
- 在产后心脏中识别和表征原生心脏前体细胞.
- 研究这些祖细胞的更新和分化潜力.
- 建立一个研究心脏细胞谱系形成和疾病的系统.
主要方法:
- 在出生后的老鼠,小鼠和人类心肌中识别ISl1+心脏原始体.
- 使用心脏介质细胞养层进行前代细胞更新.
- 采用tamoxifen诱导的Cre/lox技术进行细胞标记和净化.
- 与新生儿肌细胞进行共同培养研究,以评估分化能力.
主要成果:
- 在产后哺乳动物心肌中确定了isl1+心脏前体.
- 主细胞表现出更新能力和分化为心肌细胞 (25%),而没有细胞融合.
- 不同化的细胞表现出稳定的肌细胞标记物,完整的Ca2+循环和动作潜能生成.
- 这些细胞被证实是真正的内源性心脏原始细胞 (心脏细胞).
结论:
- 原生心脏细胞的发现为研究心脏发育提供了一个基于基因的系统.
- 这一发现为心脏细胞谱系的形成和成熟提供了机理性的见解.
- 了解这些原始体可以促进对先天性和成人心脏病的研究.
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