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Updated: Jul 11, 2026

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
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使用histidine标签控制接口上的蛋白质定向:一种替代Ni/NTA的替代方案

Daniel L Johnson1, Lisandra L Martin

  • 1School of Chemistry, Monash University, Victoria 3800, Australia.

Journal of the American Chemical Society
|February 17, 2005
PubMed
概括

研究人员开发了一种新的方法,使用宏环化剂将海斯蒂丁标记蛋白质固定在表面上. 这种方法提高了长期研究和直接电化学应用的蛋白质稳定性和特异性.

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相关概念视频

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...