活着的人类胎儿的全球基因表达分析,使用羊水中的无细胞信使RNA
Paige B Larrabee1, Kirby L Johnson, Chaoqiang Lai
1Department of Pediatrics, Division of Newborn Medicine, Tufts-New England Medical Center, Boston, Mass 02111, USA.
JAMA
|February 17, 2005
概括
研究人员使用基因表达微阵列在羊水中检测到无细胞胎儿信使RNA (mRNA). 这一突破允许监测胎儿发育,性别和疾病在体内.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 胎儿医学 胎儿医学
背景情况:
- 目前,没有分子生物学测试存在,以监测人体胎儿发育 in vivo.
- 监测胎儿发育对于早期发现异常并确保健康的妊娠结果至关重要.
研究的目的:
- 为了确定是否可以检测胚胎液中的无细胞胎儿信使RNA (mRNA).
- 研究使用寡核酸微阵列在活人胎儿中进行大规模基因表达分析的潜力.
- 根据胎儿的性别,妊娠年龄和病理学分析基因表达变异.
主要方法:
- 分析了受孕妇女 (20-32周怀孕) 的无细胞羊水样本.
- 使用来自健康对照组 (17周妊娠) 的聚合羊水.
- 使用基因表达微阵列提取,放大,标记和分析RNA.
主要成果:
- 在36%的分析的探针组中检测到无细胞胎儿mRNA.
- 在病例和对照之间观察到显著的基因表达差异 (中位数为20%).
- 发现发育转录 (例如,表面活性蛋白) 与妊娠年龄 (高达64倍) 的变化.
- 在双胞胎双胞胎输血病例中确定了增加的水载体基因转录 (高达18倍).
- 仅在男性样本中确认Y染色体转录的存在.
- 排除检测到的mRNA的胎盘起源.
结论:
- 无细胞胎儿mRNA可以成功地从羊水中提取并使用基因表达微阵列进行分析.
- 基因表达的变化是可检测的,并且与胎儿的性别,妊娠年龄和疾病状态相关.
- 这种方法提供了一个非侵入性的方法来研究胎儿的发育和病理 in vivo.
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