一种可光诱导的β-毛
Andreas Aemissegger1, Vincent Kräutler, Wilfred F van Gunsteren
1Laboratorium für Organische Chemie and Laboratorium für Physikalische Chemie, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland. hilvert@org.chem.ethz.ch
Journal of the American Chemical Society
|March 3, 2005
概括
研究人员使用光色阿佐烯链接器来控制β-hairpin的折叠. 链接器的光诱导变化使得β-hairpin结构得到了很好的定义,为的折叠和稳定性提供了洞察力.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 摄影化学的使用.
背景情况:
- β-hairpins是蛋白质中关键的二次结构.
- 控制折叠对于理解蛋白质结构功能关系至关重要.
- 光色分子提供了对分子构造的光感应控制.
研究的目的:
- 为了将光染色的阿佐烯链接器纳入β-hairpin.
- 为了研究链接器的形状对折叠的影响.
- 为了确定光是否可以用于诱导和稳定β-hairpin结构.
主要方法:
- 一种含有亚博的的合成.
- 光谱分析,包括 (1) H NMR,以确定结构.
- 光异构化研究以评估链接器动态.
主要成果:
- 亚索链体的转异构体促进了寡合体的形成,阻碍了结构的确定.
- 对cis异构体的辐射诱导了单构体的形成和一个明确的β-hairpin结构.
- 含有亚博的的异构化速率略低于未被替代的染色体.
结论:
- 光染色亚博链接剂可以作为中的可光诱导的转向元素.
- 光可以用来控制β-hairpin折叠和稳定性.
- 这种方法提供了一种研究和操纵二次结构的方法.
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