通过细胞间转移通过间隙结进行交叉呈现
Joost Neijssen1, Carla Herberts, Jan Wouter Drijfhout
1Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands. J.Neefjes@nki.nl
Nature
|March 4, 2005
概括
细胞通过间隙连接在相邻细胞之间转移,使交叉呈现成为可能. 这种细胞间转移激活了细胞毒性T细胞,影响旁观细胞和单细胞.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 主体组织相容性复合体 (MHC) 类I分子存在内源性,对T细胞反应至关重要.
- 交叉呈现允许专业的抗原呈现细胞呈现外源抗原,但抗原获取的机制尚未完全理解.
- 众所周知,细胞间通信通道,如间隙结,有助于分子直接从细胞转移到细胞.
研究的目的:
- 通过间隙连接,通过相邻细胞之间的的直接细胞间转移进行调查.
- 确定这种细胞间转移是否有助于用于交叉呈现和T细胞激活的抗原获取.
主要方法:
- 通过间隙连接通过相邻细胞之间的扩散的实验测试.
- 对细胞间传输的体大小限制的分析.
- 在细胞间酸转移后对旁观细胞和单细胞的细胞毒性T细胞识别的评估.
- 研究细胞溶液酶活性在限制转移中的作用.
主要成果:
- 高达大约1800个相对分子质量的可以通过间隙连接在细胞间扩散.
- 细胞间转移导致细胞毒性T细胞识别相邻的旁观细胞和激活的单细胞.
- 高细胞溶性酶活性限制了间隙结介导的转移到有限数量的结合细胞.
结论:
- 间隙结介导的细胞间转移为交叉呈现中的抗原获取提供了一种新的机制.
- 这个过程将邻近细胞的抗原呈现系统结合起来,可能会启动T细胞对无辜旁观者细胞的反应.
- 描述的机制与T细胞原始化有关,并且在许多瘤中似乎受损.
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