一个发光受体,在水溶液中的酸中与N端的histidine有亲和力
Michael Kruppa1, Christian Mandl, Stefan Miltschitzky
1Institut für Organische Chemie, Universität Regensburg, D-93040 Regensburg, Germany.
Journal of the American Chemical Society
|March 10, 2005
概括
这项研究介绍了一种发光传感器,用于检测氨离子和含有histidine的. 传感器显示在生理条件下具有高结合亲和力和可检测信号.
科学领域:
- 超分子化学 超分子化学
- 分析化学 分析化学
- 生物物理化学 生物物理化学
背景情况:
- 皇冠乙烯是氨离子的有效宿主,但在水溶液中缺乏亲和力.
- 开发针对生理条件的传感器需要克服低结合亲和度.
研究的目的:
- 为了合成一个发光的本佐皇冠以太传感器.
- 为了在生理pH值下能够选择性检测氨离子和含有histidine的.
- 为了恢复含有氨和伊米达的分析物在水溶液中的高结合亲和力.
主要方法:
- 一个发光的基冠以太与一个吊铜 imidodiacetic 酸复合物的合成.
- 对传感器的辐射强度变化的光谱分析.
- 在pH 7.5.5的甲醇和缓冲水溶液中测试传感器性能.
主要成果:
- 传感器显示在甲醇中含有氨离子的排放增加.
- 在pH7.5的缓冲水中,如果没有特定的分析物结构,相互作用是弱的.
- 对于含有氨和имидазол部分的分析物 (例如含有histidine的) 观察到高结合亲和度和可检测的排放增加.
结论:
- 开发的传感器在生理条件下有选择地检测N端的histidine或含有histidine的.
- 传感器的发光为绑定事件提供视觉信号.
- 这为分析水环境中的生物相关分子提供了一种新的方法.
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