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相关概念视频

Metal-Ligand Bonds02:51

Metal-Ligand Bonds

The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Protein Denaturation01:28

Protein Denaturation

The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
Complexation Equilibria: Factors Influencing Stability of Complexes01:09

Complexation Equilibria: Factors Influencing Stability of Complexes

In complexation reactions, metal cations are the electron pair acceptors, and the ligands are the electron pair donors. The stability of the metal complexes depends primarily on the complexing ability of the central metal ion and the nature of the ligands. Generally, the complexing ability of the metal ion depends on the size and charge of the ion. As the metal ion size increases, the stability of the metal complexes decreases, provided that the valency of the metal ion and the ligands remain...
Global Regulatory Systems01:28

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...

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相关实验视频

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Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
08:05

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries

Published on: June 27, 2012

金属结合的局部和全球影响在小子单位的核酸缩酶内.

Brad S Pierce1, Michael P Hendrich

  • 1Department of Chemistry, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.

Journal of the American Chemical Society
|March 10, 2005
PubMed
概括

大肠杆菌核糖核酸还原酶R2的铁结合部位表现出差异性亲和力,使独特的异核核FeMn集群成为可能. 金属结合是动力控制的,并诱导形状变化,影响后续的金属结合.

科学领域:

  • 生物化学 生物化学
  • 蛋白质结构和功能 蛋白质结构和功能
  • 酶学 是一种酶学.

背景情况:

  • 来自大肠杆菌的 рибо核酸减少酶 (R2) 含有双核铁集群,具有明显的Fe (A) 和Fe (B) 位点.
  • 了解金属位点相互作用对于酶功能和调节至关重要.

研究的目的:

  • 调查大肠杆菌中Fe (A) 和Fe (B) 位点的不同金属结合亲和力R2.
  • 探索异构核金属集群的形成以及金属结合对蛋白质构成的影响.

主要方法:

  • 无氧Fe (II) R2阿波蛋白的定位.
  • 暴露于O2或高甘油缓冲剂以改变金属职业.
  • 添加H2O2产生氧化物种.
  • 电子偏磁共振 (EPR) 谱学用于表征.

主要成果:

  • 在标准条件下,大肠杆菌R2主要连接每小单元1个Fe (II) 原子.
  • 不同的亲属性允许创建异构核 Mn(II) Fe(II) 和 Mn(III) Fe(III) 集群.
  • 金属结合是动力控制的,没有显著的位点交换.
  • 第一个Fe (II) 结合会诱导形状变化,抑制第二个位点,降低A位点的亲和力.

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Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands
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Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands

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Localized Bathless Metal-Composite Plating via Electrostamping
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Localized Bathless Metal-Composite Plating via Electrostamping

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Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
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Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries

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Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands
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Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands

Published on: March 4, 2020

Localized Bathless Metal-Composite Plating via Electrostamping
08:05

Localized Bathless Metal-Composite Plating via Electrostamping

Published on: September 22, 2020

结论:

  • 大肠杆菌R2的A和B部位表现出明显的金属结合动力学和亲和力.
  • 混合金属FeMn集群的形成是可以实现和特征的.
  • 蛋白质构造变化在调节金属融入R2中起着重要作用.