导航护送网络:通过hsp90护送介导的物理和遗传相互作用的整合地图
Rongmin Zhao1, Mike Davey, Ya-Chieh Hsu
1Department of Biochemistry, Medical Sciences Building, 1 King's College Circle, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Cell
|March 16, 2005
概括
研究人员绘制了酵母中热冲击蛋白90 (Hsp90) 护卫者的相互作用图,揭示了649个物理和遗传连接的网络. 这项研究将Hsp90与关键的细胞功能和表观遗传基因调节联系起来.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 热冲击蛋白90 (Hsp90) 是一个关键的分子伴侣,参与蛋白质折叠和稳定.
- 了解Hsp90广泛的相互作用网络对于阐明它在细胞过程中的作用至关重要.
研究的目的:
- 为了全面地绘制酵母中Hsp90的物理,遗传和化学-遗传相互作用.
- 识别新的Hsp90辅因子,了解它们的功能联系.
主要方法:
- 全基因组酵母两种混合屏幕用于物理相互作用.
- 含有Hsp90的蛋白质复合物的大规模亲和性净化.
- 合成遗传阵列 (SGA) 技术和化学遗传查使用Hsp90抑制剂geldanamycin.
主要成果:
- 构建了一个涉及Hsp90的198个物理和451个遗传/化学-遗传相互作用的广泛网络.
- 确定了两个新的Hsp90辅因子,Tah1和Pih1.
- 发现这些辅助因子与Rvb1/Rvb2 DNA螺旋酶相互作用,将Hsp90与染色体重塑和表观遗传调节联系起来.
结论:
- Hsp90与广泛的细胞蛋白相互作用,影响各种细胞功能.
- 鉴定Tah1和Pih1扩大了我们对Hsp90调节的知识.
- 这项研究确立了Hsp90与表观遗传基因调节之间的直接联系,通过其与染色质重塑机制的相互作用.
相关概念视频
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
03:00Molecular Chaperones and Protein Folding
The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
The...
02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
03:00Molecular Chaperones and Protein Folding
The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
The...
01:30Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...