碳基氨酸染料作为高效可逆单分子光学开关.
Mike Heilemann1, Emmanuel Margeat, Robert Kasper
1Applied Laserphysics & Laserspectroscopy, Physics Faculty, University of Bielefeld, Universitätsstrasse 25, 33615 Bielefeld, Germany.
Journal of the American Chemical Society
|March 18, 2005
概括
商用Cy5染料可以作为可逆光学开关. 它们的光可以在光漂白后恢复,从而使其在光学数据存储和影响生物研究中的应用成为可能.
科学领域:
- 摄影化学的使用.
- 分子生物物理学 分子生物物理学
- 材料科学 材料科学 材料科学
背景情况:
- 碳氨酸染料,如Cy5,是广泛使用的光标签.
- 光漂白限制了它们的长期稳定性和应用范围.
- 了解可逆切换是先进光学应用的关键.
研究的目的:
- 为了研究未经修改的碳酸染料的可逆光学切换能力.
- 探索光漂白后恢复光所需的条件.
- 评估这些染料在光学数据存储方面的潜力及其在生物测试中的局限性.
主要方法:
- 在水溶液中进行光漂白和回收实验.
- 固定Cy5分子的单分子光光谱学.
- 在633nm和488nm的交替激发用于光学开关.
- 在标记的寡核酸上进行光共振能量转移 (FRET) 测量.
主要成果:
- Cy5染料作为高效的可逆单分子光学开关.
- 在室温下,可以通过在较短波长 (337,488或532 nm) 的辐射来实现光恢复.
- 恢复的有效性取决于氧气的去除和三重灭剂 (例如,β-mercaptoethylamine).
- 单个Cy5分子在毫秒内切换了100多次,可靠性高于90%.
- 碳酸染料切换行为限制了它们在生物研究中作为FRET受体的使用.
结论:
- 未经修改的碳酸染料提供了一个简单,高效和可逆的光学切换机制.
- 这些染料对超高密度光学数据存储应用有前途.
- 光驱动的切换行为对使用它们作为生物研究中的能量转移受体具有根本的限制.
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