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相关概念视频

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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相关实验视频

Updated: Jul 6, 2026

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
05:48

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

Published on: May 23, 2013

微RNAs调节斑马鱼的大脑形态发生.

Antonio J Giraldez1, Ryan M Cinalli, Margaret E Glasner

  • 1Developmental Genetics Program, Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA. giraldez@saturn.med.nyu.edu

Science (New York, N.Y.)
|March 19, 2005
PubMed
概括
此摘要是机器生成的。

斑马鱼突变体缺乏成熟的microRNAs (miRNAs),影响其发育. 恢复特定的miRNAs可以挽救大脑缺陷,突出显示它们在形态发生过程中的关键作用.

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Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
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Hypoxia Alters miRNAs Levels Involved in Non-Mendelian Inheritance of Autism Spectrum Disorder in Mice
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相关实验视频

Last Updated: Jul 6, 2026

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
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Published on: May 23, 2013

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

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Hypoxia Alters miRNAs Levels Involved in Non-Mendelian Inheritance of Autism Spectrum Disorder in Mice
09:13

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科学领域:

  • 发展生物学 发展生物学
  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 微RNAs (miRNAs) 是基因表达的关键转录后调节者.
  • 迪克尔酶对于miRNA的处理和功能至关重要.
  • 了解miRNA在发育中的作用需要阻止miRNA生物发生的工具.

研究的目的:

  • 研究微RNAs (miRNAs) 在斑马鱼发育中的重要作用.
  • 描述Dicer域在miRNA生物发生和沉默中的功能.
  • 为了确定依赖于miRNAs的特定发育过程.

主要方法:

  • 产生和分析母性 - 异位性dicer (MZdicer) 斑马鱼突变体.
  • 在突变胚胎中评估miRNA处理和基因沉默.
  • 涉及注射前体miRNA和特定成熟miRNA (例如miR-430) 的救援实验.
  • 对MZdicer突变体的形态和发育分析.

主要成果:

  • MZdicer突变体无法将前体miRNA加工成成熟的miRNA.
  • 破坏的Dicer域对于miRNA沉默的后期阶段是不可或缺的.
  • 突变胚胎在形态发生过程中表现出严重的缺陷,包括大脑,体和心脏发育.
  • 注射miR-430miRNAs可挽救MZdicer突变体中的大脑发育缺陷.

结论:

  • 迪克的核糖核酶III和双链RNA结合域对于miRNA处理至关重要,但不一定适用于所有下游沉默步骤.
  • 微RNAs,特别是miR-430,在斑马鱼形态发生过程中发挥着关键和必不可少的作用.
  • 这项研究提供了一种有价值的遗传工具 (MZdicer突变),用于在体内剖析miRNA功能.