通过细胞外矩阵调节脊椎动物的左右不对称性
1Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 55455.
Nature
|May 14, 1992
概括
细胞外矩阵指导Xenopus胚胎的左右身体轴形成. 扰乱这个矩阵会破坏正常的不对称性,这表明它对器官发育具有关键的轴向信息.
科学领域:
- 发展生物学 发展生物学
- 胚胎学 胚胎学
- 细胞外矩阵研究 细胞外矩阵研究
背景情况:
- 脊椎动物的体平面依赖于三个轴:前后,背内,左右.
- 对于心脏和肠道发育至关重要的左右轴的确定性仍然不太了解.
- 在Xenopus laevis gastrulae中富含纤维素蛋白的细胞外基质 (ECM) 是迁移心脏和内脏原始体的基础.
研究的目的:
- 调查细胞外矩阵在脊椎动物发育过程中建立左右不对称性的作用.
- 确定ECM是否包含并向成长中的器官传输轴向信息.
主要方法:
- 在Xenopus laevis gastrulae中使用微手术对ECM进行局部干扰.
- 通过微注射Arg-Gly-Asp或肝酶到布拉斯托科尔中,对ECM的全球干扰.
- 在心脏和内脏发育中,ECM扰动与左右不对称的随机化相关.
主要成果:
- 局部ECM中断导致局部随机化左右不对称.
- 全球ECM中断导致左右不对称的广泛随机化.
- 这些发现表明,ECM在指导左右轴形成方面发挥着至关重要的作用.
结论:
- 细胞外矩阵包含了胚胎发育早期重要的左右轴信息.
- 这种轴向信息独立地传输到心脏和内脏原始体.
- 对于正确的左右车身模式来说,ECM完整性至关重要.
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