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相关概念视频

Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Coping with and recovering from hydroxyurea-induced replication fork arrest in budding yeast.

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Mrc1 transduces signals of DNA replication stress to activate Rad53.

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相关实验视频

Updated: Jul 17, 2026

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

在酵母复制起源的蛋白质-DNA相互作用.

J F Diffley1, J H Cocker

  • 1Imperial Cancer Research Fund, Potters Bar, Hertfordshire, UK.

Nature
|May 14, 1992
PubMed
概括

研究人员确定了两个关键蛋白质,与酵母中的DNA复制起源相互作用. 一种蛋白质是ARS结合因子1,另一种蛋白质是类似于起源识别复合体 (ORC) 的新型因子.

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 细胞生物学 细胞生物学

背景情况:

  • 了解真核细胞DNA复制起源的蛋白质-DNA相互作用对于阐明DNA合成启动和细胞周期控制至关重要.
  • 在Saccharomyces cerevisiae中的自主复制序列 (ARS) 作为DNA复制在等离子体和染色体上的起源.

研究的目的:

  • 使用核酸解析基因足迹研究ARS1复制起源的蛋白质关联.
  • 识别在ARS1.1.内部与功能元素结合的细胞因子.

主要方法:

  • 核酸分辨率基因组足迹被用来分析ARS1.1.的蛋白质-DNA相互作用.
  • 核酶保护模式被用来绘制蛋白质结合部位的地图.

主要成果:

  • 至少有两个不同的细胞因子与ARS1.1相互作用.
  • 鉴定出ARS结合因子1是一种相互作用的蛋白质.
  • 发现一种新型蛋白质可以结合ARS共识序列和侧边区域,诱导类似于ORC的扭曲应变.

结论:

  • ARS1与至少两个蛋白质因子相互作用,包括ARS结合因子1和一种新型蛋白质.

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Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

  • 观察到的新型蛋白质的结合和扭曲应变表明,在复制启动中,它与起源识别复合体 (ORC) 相似的作用.