Mnd2,在介质预相过程中,它是亚纳相促进复合体的重要对手
Alexandra M Penkner1, Susanne Prinz, Stefan Ferscha
1Vienna Biocenter II, Max Perutz Laboratories, Department of Chromosome Biology, Vienna, Austria.
Cell
|March 31, 2005
概括
Mnd2蛋白质通过调节酶促进复合体 (APC(Ama1) 来防止质变化过程中securin和Sgo1的过早降解. 这确保了正确的染色体分离,并可能为预防人类卵子细胞中形积分提供标.
科学领域:
- 细胞生物学 细胞生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 介质凝聚能确保姐妹染色体链接和DNA修复.
- 分离酶分裂凝聚的Rec8子单元,启动亚相.
- 塞库林抑制分离酶,其通过APC的降解 (Cdc20) 激活分离酶.
研究的目的:
- 研究Mnd2在调节变过程中的蛋白降解中的作用.
- 确定替代性亚纳相促进复合体 (APC) 变体在半变化I中的参与.
- 探索潜在的治疗点,以预防人类卵子细胞中的形积分.
主要方法:
- 利用mnd2Delta突变细胞观察染色体分离中的缺陷.
- 评估AMA1删除对mnd2Delta表型的影响.
- 在mnd2Delta细胞中分析了securin和Sgo1的稳定性.
主要成果:
- Mnd2 能防止在 介质变化过程中,APC ((Ama1) 过早降解蛋白质.
- mnd2Delta细胞表现出染色体轴缺陷和早熟的姐妹染色体分离.
- 删除AMA1可以抑制这些mnd2Delta相关的缺陷.
- 在mnd2Delta细胞中,Sgo1的稳定性也受到影响.
结论:
- Mnd2 起到关键的调节作用,防止在介质分裂I过程中,APC (A) 降解Sgo1和Sgo1的过早降解.
- Mnd2的功能对于保持染色体完整性和适当分离至关重要.
- 向介质性APC变异可能提供预防人类卵细胞染色体错误分离的策略.
相关概念视频
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:02Meiosis II
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...