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Mitral Valve Prolapse III: Nursing Management
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SREBP通路对固醇做出反应,并在裂变酵母中起到氧气传感器的作用
Adam L Hughes1, Bridget L Todd, Peter J Espenshade
1Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Cell
|March 31, 2005
概括
在裂变酵母中,Sre1 (胆固醇调节元素结合蛋白) 控制胆固醇合成,并激活缺氧适应基因. 这种蛋白质家族监测氧气水平,以调节对低氧条件的反应.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 固醇调节元素结合蛋白 (SREBPs) 控制哺乳动物的脂质合成.
- 在Schizosaccharomyces pombe中发现了SREBP,SCAP和Insig的同类物.
研究的目的:
- 为了研究Sre1,Scp1和Ins1在裂变酵母中的功能.
- 为了确定Sre1是否在低氧反应中起作用.
主要方法:
- 在S. pombe.中同类鉴定.
- 固醇耗尽实验. 固醇耗尽的实验.
- 微阵列分析.微阵列分析.
- 厌氧生长试验. 厌氧生长试验.
主要成果:
- Sre1通过以Scp1依赖的方式通过固醇枯竭被分裂和激活.
- Sre1激活了固醇生物合成基因.
- Sre1还激活了缺氧适应基因,并且对于无氧生长是必需的.
结论:
- Sre1和Scp1监测依赖氧气的固醇合成,作为氧气供应的间接指标.
- 在裂变酵母中,Sre1调解了缺氧反应.
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