表皮摩尔芬:一种中细胞蛋白质,对于表皮形态发生是必不可少的
Y Hirai1, K Takebe, M Takashina
1Biomaterial Research Institute, Yokohama, Japan.
Cell
|May 1, 1992
概括
一种新发现的蛋白质,表观素,对于表皮形态发生至关重要. 这种细胞表面分子在胚胎发育过程中调解了必要的上皮细胞-介质细胞相互作用.
科学领域:
- 发育生物学 发展生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 表皮细胞形态发生是一种复杂的过程,涉及表皮细胞和介质细胞之间的协调相互作用.
- 了解控制这些相互作用的分子机制对于发育生物学和再生医学至关重要.
研究的目的:
- 识别和描述涉及表皮-介质细胞相互作用的新型分子.
- 阐明一种新发现的蛋白质在调节上皮质形态发生过程中的作用.
主要方法:
- 来自小鼠胚胎介质细胞的新型150 kDa细胞表面蛋白的鉴定和特征.
- 产生单克隆抗体以抑制器官培养中的蛋白质功能.
- cDNA测序和转化为NIH 3T3细胞.
- 与肺上皮细胞和转染的NIH 3T3细胞进行共同培养实验.
主要成果:
- 在介质细胞表面上发现了一种新型的150kDa蛋白质 - - epimorphin.
- 单克隆抗体对抗表观素抑制了毛囊生长和肺上皮管管状形成 in vitro.
- 通过对NIH 3T3细胞感染epimorphin cDNA,导致蛋白质的表面表达.
- 与表达表观素的NIH 3T3细胞共同培养恢复了正常的肺上皮质管状形态发生.
结论:
- 乙摩尔芬是表皮质-介质细胞相互作用的关键媒介.
- 这种蛋白质在胚胎发育期间调节上皮质形态发生过程中发挥着关键作用.
- 爱皮莫尔芬代表了理解和操纵发育过程的潜在目标.
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