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编码GAP相关蛋白p190的cDNA分子克隆:从ras到核的信号通路的含义
J Settleman1, V Narasimhan, L C Foster
1Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142.
Cell
|May 1, 1992
概括
研究人员确定了一种蛋白质,p190,在刺激细胞中与ras GTPase激活蛋白 (GAP) 复合. 这种蛋白质具有域,表明其在GTPase调节和转录抑制中的作用,可能将ras信号与核基因表达联系起来.
科学领域:
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
- 遗传学 是一个遗传学.
背景情况:
- 拉斯GTPase激活蛋白 (GAP) 在调节拉斯信号通路方面发挥着至关重要的作用.
- 观察到p190蛋白在刺激和转化细胞中与ras GAP形成复合体.
研究的目的:
- 为了克隆和表征p190蛋白质.
- 阐明p190在细胞信号传输中的功能领域和潜在作用.
主要方法:
- 克隆编码p190蛋白的互补DNA (cDNA).
- 对预测的蛋白质序列进行生物信息分析,以识别同源域.
主要成果:
- 蛋白质序列p190包含三个不同的域,与已知蛋白质具有显著的同质性.
- 一个N端域显示同质性GTPase动机.
- C终端域与已知的GAP蛋白相似,中心域与转录抑制剂相同.
结论:
- p190具有结构特征,表明在GTPase调节和转录抑制中具有双重功能.
- p190可能充当信号媒介,将ras GTPase激活蛋白的信号转导到核中.
- 这种相互作用可能会影响特定细胞基因的表达,影响细胞过程.
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