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Updated: Jun 25, 2026

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
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High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

Published on: January 22, 2019

在无细胞转化系统中对与HIV-1相关的CCR5序列进行双重催化传感,使用可控制的核糖调节器控制的光酶活性

Shinsuke Sando1, Atsushi Narita, Kenji Abe

  • 1Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.

Journal of the American Chemical Society
|April 14, 2005
PubMed
概括

这项研究引入了一种新型的催化基因传感方法,使用肋骨调节器来检测特定的DNA或RNA序列. 该系统实现了高灵敏度和单核酸分辨率用于目标检测.

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Measuring Endoplasmic Reticulum Stress and Unfolded Protein Response in HIV-1 Infected T-Cells and Analyzing its Role in HIV-1 Replication

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Retrovirus Life Cycles01:10

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...