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相关概念视频

Retrovirus Life Cycles01:10

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...

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High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
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Published on: January 22, 2019

在无细胞转化系统中对与HIV-1相关的CCR5序列进行双重催化传感,使用可控制的核糖调节器控制的光酶活性.

Shinsuke Sando1, Atsushi Narita, Kenji Abe

  • 1Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.

Journal of the American Chemical Society
|April 14, 2005
PubMed
概括

这项研究引入了一种新型的催化基因传感方法,使用肋骨调节器来检测特定的DNA或RNA序列. 该系统实现了高灵敏度和单核酸分辨率用于目标检测.

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科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 生物感应是一种生物感应.

背景情况:

  • 催化基因传感提供了扩大核酸的检测.
  • 肋骨调节器可以通过标结合来控制基因表达.
  • 对于某些目标,现有的方法可能缺乏敏感性或特异性.

研究的目的:

  • 开发一种多种催化基因传感系统,使用分子信标类型的核糖调节器.
  • 为了能够对人类CC化学受体5 (CCR5) 序列进行敏感和特定的检测.
  • 为了证明该系统在无细胞转化系统中的适用性.

主要方法:

  • 构建了一个带有记者基因 (火 luciferase) 和一个目标-补充循环的肋骨调节器.
  • 用一种无细胞转化系统来进行转录和翻译.
  • 通过催化化学发光反应测试光酶活性.
  • 使用未经修改的RNA或双链DNA作为探针.

主要成果:

  • 肋骨调节器系统成功检测到目标的寡核酸序列.
  • 对于目标序列,达到低于或等于50 fmol的灵敏度.
  • 在目标检测中证明了单核酸分辨率.
  • 该系统表现出双催化放大,增强信号输出.

结论:

  • 开发的肋骨调节器系统为基因传感提供了敏感和特定的方法.
  • 这种多重催化方法为核酸检测提供了显著的优势.
  • 该系统与RNA或DNA探针的适应性扩大了其潜在的应用.