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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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相关实验视频

Updated: Jul 9, 2026

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
09:49

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

Published on: July 14, 2014

用硫酸覆盖的金纳米颗粒的功能化,用于特定地固定用histidine标记蛋白质.

José M Abad1, Stijn F L Mertens, Marcos Pita

  • 1Instituto de Catálisis y Petroleoquímica, CSIC, C/Marie Curie 2, 28049 Madrid, Spain. jmabad@icp.csic.es

Journal of the American Chemical Society
|April 14, 2005
PubMed
概括

这项研究引入了一种新的方法,用于将功能性蛋白质附加到纳米粒子上,使用终端外和胺标签. 这确保了特定蛋白质的固定,没有不必要的吸附,保持酶活性.

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Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
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Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

Biofunctionalization of Magnetic Nanomaterials
06:40

Biofunctionalization of Magnetic Nanomaterials

Published on: July 16, 2020

相关实验视频

Last Updated: Jul 9, 2026

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
09:49

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

Published on: July 14, 2014

Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

Biofunctionalization of Magnetic Nanomaterials
06:40

Biofunctionalization of Magnetic Nanomaterials

Published on: July 16, 2020

科学领域:

  • 生物结合化学 生物结合化学
  • 纳米技术纳米技术
  • 蛋白质工程是指蛋白质工程.

背景情况:

  • 功能性蛋白质在纳米材料上的特定固定对于生物传感和生物催化中的应用至关重要.
  • 现有的方法经常受到非特异性吸附或蛋白质活性丧失的影响.
  • 开发针对蛋白质附着的有针对性的策略仍然是一个重大挑战.

研究的目的:

  • 开发一种有效和具体的策略,将功能齐全的蛋白质固定在纳米粒子表面上.
  • 为了利用 (II) 终结的连接体外和基因工程的histidine标签用于向蛋白质的附着.
  • 用模型酶来证明这种方法的有效性.

主要方法:

  • 使用硫酸衍生黄金集群作为阶段性修改的支架.
  • 在纳米粒子表面上创建一个 ((II) 终结的连接体外.
  • 协调基因工程蛋白质与 (II) 离子的胺标签.
  • 使用缺乏histidine标签的酶类似物验证固定化特异性.

主要成果:

  • 成功地证明了功能性胡卜Peroxidase和ferredoxin-NADP(+) 减少酶在Co(II) 终端纳米颗粒上的特定固定.
  • 证实固定蛋白质保留了它们的全部功能.
  • 展示了在纳米界面上缺乏非特异性蛋白质吸附.
  • 通过对照实验验证结合部位的特异性.

结论:

  • 开发的策略提供了一种高效和高度特定的方法,用于将功能性蛋白质固定在纳米粒子上.
  • 这种方法最大限度地减少了非特异性结合,并保持了蛋白质活性,为基于纳米材料的先进应用开辟了道路.
  • 希斯蒂丁标记-金属离子协调提供了一个强大的平台,用于蛋白质-纳米粒子结合.