来自Methylococcus capsulatus (Bath) 的可溶性甲单氧基酶基酶的产品结合结构:α子单元中的蛋白质运动
Matthew H Sazinsky1, Stephen J Lippard
1Contribution from the Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Journal of the American Chemical Society
|April 21, 2005
概括
可溶性甲单氧化酶氧化酶 (MMOH) 酶.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 酶学 是一种酶学.
背景情况:
- 可溶性甲单氧化酶氧化酶 (MMOH) 的α子单元具有对基质/产品运输到其二铁中心至关重要的空腔.
- 腔的结构差异区分MMOH (甲-基化) 和ToMOH (非甲-基化).
研究的目的:
- 阐明MMOH腔的功能,以容纳各种基板.
- 为了研究MMOH如何适应氧化甲以外的大分子.
主要方法:
- 用各种酒精浸泡的MMOH晶体的X射线晶体学.
- 高分辨率结构分析 (1.8-2.4 A) 以观察连接体结合和结构变化.
主要成果:
- 产品类似物 (酒精) 定位在 1-3 腔,揭示了基质入口/产品出口通道.
- 在二铁中心的酒精结合验证了其在基质氧化中的作用.
- 观察到洞穴扰动和通道形成,类似于ToMOH.
- -1--1-的结合诱导了α子单元的四螺旋捆中的二次结构重排.
结论:
- MMOH腔结构有助于基板的接入和产品的释放.
- 酶的灵活性,包括二次结构的重新排列,使得更大的基板可以容纳.
- 这些发现对了解MMOH的机制及其效应蛋白MMOB的作用有影响.
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