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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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相关实验视频

Updated: Jul 6, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

使用银色超级镜头进行低衍射有限光学成像.

Nicholas Fang1, Hyesog Lee, Cheng Sun

  • 15130 Etcheverry Hall, Nanoscale Science and Engineering Center, University of California, Berkeley, CA 94720-1740, USA.

Science (New York, N.Y.)
|April 23, 2005
PubMed
概括
此摘要是机器生成的。

研究人员制造了一种银色超级镜头,用于低衍射限制成像,达到60纳米分辨率. 这一突破使得纳米结构的高保真性成像成为可能,为先进的光学设备打开了大门.

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Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

相关实验视频

Last Updated: Jul 6, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
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Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

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科学领域:

  • 光学和光子学 在光学和光子学.
  • 材料科学 材料科学 材料科学
  • 纳米技术纳米技术

背景情况:

  • 理论预测表明,超级镜头可以克服成像的衍射极限.
  • 对于次衍射成像至关重要的发光波通常会丢失.
  • 表面等离子激发是一种用于 evanescent 波回收的拟议机制.

研究的目的:

  • 用一个实用的超级镜头来演示子衍射有限成像.
  • 为了实现纳米结构的高分辨率成像.
  • 探索超级镜头在纳米尺度成像和设备中的潜力.

主要方法:

  • 使用银作为天然光学超级镜头材料.
  • 采用表面等离子激发来恢复 evanescent 波.
  • 优化工作波长和银厚度用于成像纳米结构.

主要成果:

  • 实现了具有60纳米半角分辨率的次衍射有限成像.
  • 证明分辨率为照明波长的六分之一.
  • 通过精心设计展示了任意纳米结构的高保真成像.

结论:

  • 银色功能作为一个有效的光学超级镜头用于亚衍射成像.
  • 超级镜头技术使得比衍射极限小的特征能够被成像.
  • 这一进步对纳米级光学成像和超微型光电子设备具有前景.