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在植物中转基因作用siRNA生物发生过程中,microRNA导向分相
Edwards Allen1, Zhixin Xie, Adam M Gustafson
1Center for Gene Research and Biotechnology, Oregon State University, Corvallis, Oregon 97331, USA.
Cell
|April 27, 2005
概括
微RNAs (miRNAs) 指导植物中转化作用siRNAs (ta-siRNAs) 的处理,揭示了一个新的调节机制. 这种miRNA引导的裂变启动ta-siRNA生物发生,影响基因调节.
科学领域:
- 植物分子生物学 植物分子生物学
- RNA生物学的RNA生物学
- 基因调节 基因调节
背景情况:
- 植物和动物利用小RNA,包括微RNA (miRNA) 和小干扰RNA (siRNA),进行基因调控.
- 对于植物miRNA和转基因作用siRNA (ta-siRNA) 存在着不同的生物发生路径,尽管这两种都指导着转录裂变.
研究的目的:
- 使用综合方法识别Arabidopsis thalianamiRNAs和ta-siRNAs的目标.
- 阐明miRNAs和ta-siRNA生物发生之间的调节关系.
主要方法:
- 综合分析以确定Arabidopsis thaliana中的小RNA标.
- 研究特定miRNAs (miR173,miR390) 在ta-siRNA前体处理中的作用.
主要成果:
- 确定了几种新的小型RNA调节基因类,包括Argonaute2和E2-ubiquitin结合酶.
- 发现五个ta-siRNA生成转录是miR173或miR390.0的目标.
- 通过miRNA引导的ta-siRNA前体的分裂被证明可以建立ta-siRNA生成的阶段性处理.
结论:
- 提出了一个ta-siRNA生物发生的新型模型,其中miRNA引导的裂变启动了这个过程.
- 这种依赖miRNA的机制会产生分阶段ta-siRNAs,后续调节其他基因.
- 这项研究揭示了一层复杂的基因调节层,涉及植物中miRNAs和ta-siRNAs之间的相互作用.
相关概念视频
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

