一个DNA复制的起源和一个转录静音器需要一个共同的元素
1Department of Molecular and Cell Biology, University of California, Berkley 94720.
Science (New York, N.Y.)
|May 1, 1992
概括
研究人员在酵母中发现了一种DNA元素,它既起到复制来源的作用,也起到沉声器的作用. 这一特定部位对于启动染色体复制和调节基因转录至关重要.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学是一种遗传学.
- 酵母生物学的酵母生物学
背景情况:
- 细胞DNA复制始于特定的起源.
- 基因转录是通过像沉声器这样的DNA元素来调节的.
- 麦芽的Saccharomyces cerevisiae HMR-E位点含有一个已知的沉声器元素.
研究的目的:
- 为了识别和描述真核细胞的染色体起源的复制.
- 调查复制起源和转录调控元素之间的关系.
主要方法:
- 原点的地图位置分析.原点的地图位置分析.
- 对HMR-E安声器的删除分析.
- 合成短发的突变发生用于和突变发生.
主要成果:
- 在Saccharomyces cerevisiae中确定了复制的真核染色体起源.
- 这种来源与HMR-E静音器共同定位.
- 对于复制启动和转录抑制,HMR-E静音器内的特定位置至关重要.
结论:
- 细胞染色体复制的启动取决于特定的DNA序列元素.
- 一个单一的DNA元素可以同时调节DNA复制和基因转录.
相关概念视频
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02:05DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
01:44Complementary DNA
Overview
02:05DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...