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相关概念视频

The DNA Helix01:16

The DNA Helix

Overview
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...

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相关实验视频

Updated: Jul 6, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

在DNA中含有水解自身抗体.

A M Shuster1, G V Gololobov, O A Kvashuk

  • 1V.A. Engelhardt Institute of Molecular Biology, Academy of Sciences of Russia, Moscow.

Science (New York, N.Y.)
|May 1, 1992
PubMed
概括

患有自身免疫性疾病的患者的自身抗体表现出DNA-nicking活动. 这种DNA水解活性被称为免疫球蛋白M (IgM) 和免疫球蛋白G (IgG),与标准脱氧核糖核酶 (DNase) 不同.

科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 患有自身免疫病理的患者可以表现出独特的血清活动.
  • 自体抗体是自身免疫性疾病的关键参与者.
  • 自体抗体的酶活性尚未完全表征.

研究的目的:

  • 调查自身免疫性疾病患者血清中DNA-nicking活动的存在和性质.
  • 为了描述负责DNA水解的自身抗体.
  • 将DNA降解模式与已知的脱氧核糖核酶 (DNases) 进行比较.

主要方法:

  • 从患有自身免疫病理的患者的血清样本中分析了DNA-nicking活动.
  • afinity 和高性能液态色谱被用于净化.
  • 免疫球蛋白M (IgM) 和免疫球蛋白G (IgG) 被确定为活性成分.
  • 评估了酸冲击稳定性和DNA降解模式.

主要成果:

  • 在患者血清中检测到DNA-nicking活动,并归因于自身抗体.
  • 净化的活性与IgM和IgG相对应,与抗人类IgG抗体发生反应.
  • 这些DNA水解自抗体表现出对酸性冲击的稳定性.

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Extraction of Diatom DNA from Water Samples and Tissues
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Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

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Extraction of Diatom DNA from Water Samples and Tissues

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  • 这些自身抗体产生的DNA降解模式与DNase I和血液DNase不同.

    • 结论:

    • 自身免疫性疾病中的自身抗体可能具有DNA水解能力.
    • 已识别的自身抗体是具有核酶类活性的免疫球蛋白 (IgM和IgG).
    • 这种活动与内源性DNase不同,并表现出独特的稳定性.