在Dictyostelium中的结尾是由cAMP依赖的蛋白激酶调节的
A J Harwood1, N A Hopper, M N Simon
1Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, England.
Cell
|May 15, 1992
概括
在Dictyostelium discoideum前茎细胞中抑制循环AMP依赖蛋白激酶 (PKA) 延长了的迁移并阻止了茎细胞的分化. PKA活动对于调节细胞运动和在结尾期间的分化至关重要.
科学领域:
- 细胞和分子生物学 细胞和分子生物学
- 发育生物学 发展生物学
- 生物化学 生物化学
背景情况:
- 细胞分化和形态发生是通过信号通路调节的复杂过程.
- 迪西奥斯蒂 (Dictyostelium discoideum) 作为研究多细胞发育的模型生物.
- 循环AMP依赖蛋白激酶 (PKA) 在各种细胞过程中发挥作用,包括发育.
研究的目的:
- 为了研究PKA活动在前细胞分化和Dictyostelium discoideum的高潮中的作用.
- 确定PKA对于调节细胞运动和分化在高潮阶段的必要性.
主要方法:
- 一个特定的PKA抑制剂被置于Dictyostelium discoideum中一个前特异性促进物的控制之下.
- 在通常引发结尾的环境条件下观察到突变的.
- 进行了与野生类型细胞的共同开发测试.
- 通过DIF (差异化诱导因子),茎细胞形态原,评估了诱导性.
主要成果:
- 突变的牛表现出长期的迁移,而不是立即的高潮.
- 达到顶点,一旦启动,就会产生长长的结构,缺乏茎和子细胞.
- 突变细胞在与野生类型细胞共同发展时,向茎入口的迁移减少.
- 在突变细胞中,DIF的诱导性显著降低.
结论:
- 在结尾过程中,PKA活动对于预茎细胞的特征性细胞运动至关重要.
- 对于茎细胞的分化,PKA是必要的.
- 提出了一个模型,其中PKA调节的抑制器控制了DIF诱导的茎细胞分化,平衡迁移和结尾.
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