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Updated: Jul 5, 2026

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
12:13

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Published on: October 8, 2012

在Flp重组过程中,活点氨酸在trans中的DNA裂变:在交换链之前切换蛋白质合作伙伴

J W Chen1, J Lee, M Jayaram

  • 1Department of Microbiology, University of Texas, Austin 78712.

Cell
|May 15, 1992
PubMed
概括

Flp重组酶使用四个单体和关键残留物,如Tyr-343用于DNA重组. 有效的催化需要对这些残留物进行特定的安排,使得成功的基因交换能够实现跨裂变.

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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

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