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转化控制的新范式:通过Bicoid和eIF4E相关的4EHP通过5'-3'mRNA连接进行抑制

Park F Cho¹, Francis Poulin, Yoon Andrew Cho-Park

¹Department of Biochemistry, McGill Cancer Center, McGill University, 3655 Promenade Sir William Osler, Montréal, Québec H3G 1Y6, Canada.

|May 11, 2005

概括

比科伊德 (Bcd) 蛋白质使用d4EHP,一个结蛋白,以抑制Drosophila胚胎中的尾部 (cad) mRNA转化. 这种由cad 3'非翻译区域介导的相互作用建立了翻译控制的新模型.

科学领域:

分子生物学分子生物学
发育生物学发展生物学
遗传学是一个遗传学.

背景情况:

转化控制对于细胞生长,生物体发育和胚胎模式至关重要.
mRNA 5'帽子识别是调节翻译的常见目标.
在Drosophila中,Bicoid (Bcd) 在前面抑制尾部 (cad) mRNA转化,从而产生蛋白质不对称.

研究的目的:

调查比科伊德 (Bcd) 抑制尾部 (cad) mRNA转换的机制.
为了确定eIF4E相关蛋白d4EHP在这个过程中的作用.
阐明一个新的范式的上限依赖的翻译控制.

主要方法:

共同免疫沉检测蛋白质相互作用.
记者测试以测量翻译活动.
对mRNA 3'未翻译区域 (UTR) 的分析.

主要成果:

d4EHP 特别与 Bcd 相互作用.
在cad 3' UTR中,BCd与BCd结合区域 (BBR) 的结合对于转化抑制至关重要.
同时的d4EHP与mRNA盖和BCd与BBR的相互作用导致翻译抑制.

结论:

d4EHP充当了支架,将BCd与mRNA盖复合体连接起来,以抑制翻译.
这种机制代表了一种新的上限依赖的翻译控制形式,不涉及正规的eIF4E.
这些发现揭示了一个新的范式,涉及mRNA5'和3'末端的绑定用于翻译调节.

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