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相关概念视频

Karyotyping01:17

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Gene Duplication and Divergence02:37

Gene Duplication and Divergence

The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was  generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are characterized.
Genetic Variation01:25

Genetic Variation

Genetic variation is the diversity in DNA sequences found among individuals of the same species. This diversity is crucial for a species' survival because it helps organisms adapt to environmental changes. Genetic variation begins with fertilization, where an egg and sperm cell merge. Each of these cells carries 23 chromosomes, up to 46 in the fertilized egg. Chromosomes are long DNA strands that contain genes, the basic units of heredity.
Genes exist in different versions called alleles, which...

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相关实验视频

Updated: Jul 8, 2026

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

在光染色基基因中进行状差异化.

Tony J Wigglesworth1, David Sud, Tyler B Norsten

  • 1Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada.

Journal of the American Chemical Society
|May 19, 2005
PubMed
概括
此摘要是机器生成的。

化滴乙烯衍生物经受立体选择性光诱导循环,产生单个具有高纯度的二聚体. 光诱导的转换导致光学旋转的显著变化,显示出光响应性行为.

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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

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相关实验视频

Last Updated: Jul 8, 2026

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
13:06

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Published on: December 15, 2012

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
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科学领域:

  • 有机化学 有机化学
  • 摄影化学的使用.
  • 立体化学是一种立体化学.

背景情况:

  • 乙乙烯衍生物以其光色特性而闻名.
  • 奇拉辅助剂可以影响光化学反应的立体化学结果.

研究的目的:

  • 为了研究一种性滴乙烯衍生物的立体选择性光诱导循环.
  • 为了探索由此产生的二聚体的光响应光学特性.

主要方法:

  • 一种dithienylethene衍生物的合成,该衍生物与基拉皮基臂功能化.
  • 使用特定波长 (400 nm和> 434 nm) 诱导循环化的光辐射实验.
  • 通过使用诸如奇拉色谱和光学旋转测量等技术分析二聚体纯度.

主要成果:

  • 光诱导循环反应产生了一种单一的二聚体,其纯度超过98%.
  • 在交替光照射后,观察到光学旋转的显著变化 (高达Delta[alpha]373 = 8698度).
  • 立体化学结果受到高度控制,有利于形成一种特定的二聚体.

结论:

  • 基拉尔烯基臂有效地指导了立体选择性光诱导的dithienylethene的循环.
  • 该分子的光响应性允许通过光操纵来调整可调的光学特性.
  • 这项研究展示了一种有希望的方法,用于创建可光切换的性材料.