自可以调节植物天生的免疫反应期间的细胞死亡
Yule Liu1, Michael Schiff1, Kirk Czymmek2
1Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520.
Cell
|May 24, 2005
概括
植物BECLIN 1在对感染部位的过敏反应 (HR) 期间限制了编程细胞死亡 (PCD). 这种必不可少的自途径组成部分防止了广泛的细胞死亡,保持了植物免疫力.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 植物天生的免疫反应涉及过敏反应 (HR),是一种编程细胞死亡 (PCD).
- 有效的HR要求PCD仅限于感染部位,以防止病理后果.
- 调节HR PCD局部化的精确机制尚未完全理解.
研究的目的:
- 调查植物BECLIN 1,一种与自相关的基因在调节HR PCD中的作用.
- 为了确定BECLIN 1是否能够将PCD限制在感染部位.
- 为了阐明自途径在植物天生的免疫力中的参与.
主要方法:
- 对BECLIN 1缺乏的植物突变物的分析.
- 在接种和远端组织中HR PCD的表型特征.
- 在HR期间评估自诱导.
- 调查其他自相关基因 (PI3K/VPS34,ATG3,ATG7) 在HR PCD限制中的需求.
主要成果:
- 缺乏BECLIN 1的植物正常启动HR PCD,但在相邻和远端组织中表现出不受控制的细胞死亡.
- 在HR期间,自在受感染和未受感染的细胞中都被诱导,在BECLIN 1缺乏的植物中诱导率降低.
- 限制HR PCD取决于PI3K/VPS34,ATG3和ATG7的正义基因,这表明自途径的作用.
结论:
- 植物BECLIN 1对于将HR PCD限制在感染部位至关重要.
- 进化保存的自途径在植物天生的免疫力中起着至关重要的作用.
- 自会负面调节PCD,防止植物免疫反应期间的过度细胞死亡.
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