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相关概念视频

Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...

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In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model
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Smad1保护心肌细胞免受缺血-再输液损伤的影响.

Mitsuru Masaki1, Masahiro Izumi, Yuichi Oshima

  • 1Department of Molecular Medicine, Osaka University Graduate School of Medicine, 2-2, Yamadaoka, Suita City, Osaka 565-0871, Japan.

Circulation
|May 25, 2005
PubMed
概括

Smad1信号通路通过减少心肌细胞亡来保护成人心脏免受缺血-再输液 (I/R) 损伤. 在小鼠中过度表达Smad1,在I/R事件后显著减少心脏损伤和细胞死亡.

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科学领域:

  • 心血管生物学 心血管生物学
  • 分子心脏病学分子心脏病学
  • 细胞信号传输 细胞信号传输

背景情况:

  • 骨形态遗传蛋白2 (BMP2) 之前已经在血清缺乏心肌细胞中表现出对血清丧失的心脏保护作用.
  • 这种保护是由通过Smad1信号通路诱导Bcl-xL介导的.
  • 目前的研究调查了Smad1在压力下促进成人心脏细胞存活中的作用.

研究的目的:

  • 为了确定Smad1信号是否促进成人心脏中的细胞存活.
  • 在体内调查Smad1在保护免受缺血-再输液 (I/R) 损伤方面的作用.
  • 阐明Smad1介导心脏保护背后的分子机制.

主要方法:

  • 研究了BMP2和Smad1对大鼠新生儿心肌细胞在低氧-重氧化期间心肌细胞存活率的影响.
  • 使用alpha-myosin重链促进剂生成具有Smad1 (Smad1TG) 心脏特异过度表达的转基因小鼠.
  • 接受Smad1TG和野生型 (WT) 老鼠的I / R损伤 (1小时的绑定,1小时的再注射) 并使用TUNEL和DNA梯子测定评估心肌梗塞和心肌细胞亡.

主要成果:

  • 在体外,BMP2和Smad1都显著提高了心肌细胞存活率,并减少了细胞亡.
  • 在正常小鼠心脏中,Smad1在I/R期间被激活,Smad1TG心脏显示化Smad1.1.
  • 与I/R损伤后的WT小鼠相比,Smad1TG小鼠表现出显著较小的心肌梗塞大小和减少的心肌细胞亡.
  • 在I/R后的Smad1TG心脏中观察到Bcl-xL和β-catenin的表达增加和caspase-3激活的减少.

结论:

  • Smad1信号通路在心脏保护中起着至关重要的作用,防止缺血-再输液 (I/R) 损伤.
  • Smad1的激活赋予了对I/R诱导的心肌细胞死亡的抵抗力.
  • 向Smad1通路可能是缓解缺血事件引起的心脏损伤的治疗策略.