心脏中线粒体腺核酸的发生后复苏
G K Asimakis1, J B Zwischenberger, K Inners-McBride
1Department of Surgery, University of Texas Medical Branch, Galveston 77550.
Circulation
|June 1, 1992
概括
在心脏缺血期间耗尽的线粒体腺核酸 (AdN) 在重输后1-7天内恢复. 这种恢复先于改善呼吸和转位酶功能,这表明ADN补充是心脏恢复的关键.
科学领域:
- 心脏病学 心脏病学
- 线粒体生物学 线粒体生物学
- 生物化学 生物化学
背景情况:
- 线粒体腺核酸 (AdN) 对心脏能量代谢至关重要.
- 缺血导致心脏线粒体的ADN损失,但回后的恢复情况尚不清楚.
研究的目的:
- 为了研究一段时间的缺血和再输血后,犬心中的线粒体ADN池的恢复.
主要方法:
- 狗心经历了30分钟的LAD封闭,随后有不同的再输血时间 (0,30分钟,1天,7天).
- 量化了线粒体和组织的ADN水平 (ATP,ADP,AMP).
- 评估了线粒体功能 (呼吸,腺核酸转位酶活性).
主要成果:
- 线粒体ADN水平在再注射1至7天之间显著恢复.
- 组织ADN水平也显示恢复,与线粒体ADN含量良好相关.
- 线粒体呼吸功能仅在没有回流的组中受损,而转位酶活性没有受到影响.
结论:
- 线粒体ADN的缺血后恢复发生在重灌后1至7天之间.
- 线粒体呼吸和腺因核酸转位酶功能的恢复在完全恢复AdN池之前.
- 早期再注射期间线粒体ADN水平较低并不完全解释后缺血性收缩功能障碍.
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