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相关概念视频

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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相关实验视频

Updated: Jul 14, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

斑马鱼胚胎发育中的微RNA表达

Erno Wienholds1, Wigard P Kloosterman, Eric Miska

  • 1Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, the Netherlands.

Science (New York, N.Y.)
|May 28, 2005
PubMed
概括

微RNAs (miRNAs) 调节基因表达,对胚胎发育至关重要. 在斑马鱼中,大多数miRNA在发育晚期是组织特异的,这表明它们在分化中的作用,而不是最初的组织形成.

科学领域:

  • 发育生物学 发展生物学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 微RNAs (miRNAs) 是调节基因表达的小型非编码RNAs.
  • 通过miRNA介导的基因调节对于胚胎发育至关重要.
  • 了解miRNA表达模式是解读它们的发育角色的关键.

研究的目的:

  • 研究斑马鱼胚胎中保存的脊椎动物miRNAs的时间和空间表达.
  • 阐明miRNAs在胚胎发育早期和后期阶段的作用.

主要方法:

  • 使用微阵列来分析miRNA的表达.
  • 采用现场杂交与锁定核酸 (LNA) 探针进行精确的定位.
  • 分析了115个保存的脊椎动物miRNA的表达模式.

主要成果:

  • 大多数miRNA都表现出高度组织特异的表达模式.
  • 这种组织特异性在细分和后来的发育阶段被观察到.
  • 早期的发育阶段显示了最小的miRNA表达.

结论:

  • miRNA表达在很大程度上受限于斑马鱼胚胎发生的后期阶段.

更多相关视频

In ovo Expression of MicroRNA in Ventral Chick Midbrain
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Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
08:37

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

相关实验视频

Last Updated: Jul 14, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

In ovo Expression of MicroRNA in Ventral Chick Midbrain
09:19

In ovo Expression of MicroRNA in Ventral Chick Midbrain

Published on: September 16, 2013

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
08:37

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

  • 这些发现表明miRNAs主要参与组织分化或保持身份,而不是初始组织命运的建立.
  • 这项研究提供了关于miRNAs在脊椎动物发育过程中的功能作用的见解.