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在蛋白质介质中对α-氨基酸进行色度测量,以实现对α-氨基酸的反分辨

J Frantz Folmer-Andersen1, Vincent M Lynch, Eric V Anslyn

  • 1Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA.

Journal of the American Chemical Society
|June 2, 2005
PubMed
概括

这项研究引入了一种新的色度测量方法,用于确定α-氨基酸的光学纯度. 该试验使用性铜 (II) 主体进行精确的反体差异化,无需复杂的样本准备.

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.