由细胞外醇/二硫化物氧化还原状态调节的细胞内动事件和细胞粘附
1Department of Medicine, Divisions of Cardiology and Pulmonary, Emory University, Atlanta, Ga 30322, USA.
Circulation
|June 2, 2005
概括
氧化压力有助于动脉样硬化. 该研究表明,血中的囊/囊 (Cys/CySS) 氧化还原潜力调节了早期动脉样硬化发展,这表明它可能是血管疾病风险标志物.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 血管生物学 血管生物学
背景情况:
- 氧化应激是动脉样硬化发展的一个已知的因素.
- 生物醇,如氨酸 (Cys) 和其二硫化氨酸 (CySS),在压力下被氧化.
- 细胞外Cys/CySS氧化还原潜力的早期动脉样硬化的作用尚不清楚.
研究的目的:
- 研究细胞外氨酸/氨酸 (Cys/CySS) 氧化还原潜力 (E(h)) 在调节早期动脉样硬化中的作用.
- 为了确定Cys/CySS氧化是否因果导致动脉样硬化发展.
主要方法:
- 分析了Cys,CySS,谷氨 (GSH) 和GSSG水平,以计算人体血中的E (h).
- 培养的内皮细胞和单细胞暴露于不同的细胞外E (h) 水平.
- 评估了H2O2和氧化的产生,核因子-kappaB的激活和粘附分子的表达.
主要成果:
- 氧化Cys/CySS E(h) 刺激过氧化 (H2O2) 的产生,激活核因子-kappaB,并增加粘附分子的表达.
- 氧化E (h) 促进单细胞对内皮细胞的粘附.
- 细胞外E (h) 直接影响了细胞表面醇/二硫化物还氧化状态和H2O2生产.
结论:
- 细胞外Cys/CySS氧化还原潜力对于调节早期动脉样硬化至关重要.
- Cys/CySS E (h) 可能作为血管疾病风险的潜在生物标志物.
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