Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Internal and External Forces01:12

Internal and External Forces

Newton's first law states that a net external force causes a change in motion. External forces act on an object or system, originating outside of the object or system. In contrast, internal forces originate inside the system of interest and do not lead to any acceleration. In simpler words, internal forces are forces that act on one part of an object and are exerted by another part of the same object. External forces are forces that act on an object due to some other object. Therefore, when...
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
Whole Body Regeneration01:33

Whole Body Regeneration

Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential; even...
External and Internal Respiration01:24

External and Internal Respiration

External respiration occurs in the lungs, and it is the first step in the journey of oxygen inside the body. When we inhale, oxygen enters our lungs and diffuses across the thin alveolar membrane. The alveoli are tiny, air-filled sacs that provide a vast surface area for gas exchange. Oxygen in the alveoli has a higher partial pressure (105 mmHg) than in the adjacent pulmonary capillaries (40 mmHg), establishing a pressure gradient. As a result, oxygen molecules move from the alveoli into the...
External Female Genitals01:15

External Female Genitals

The vulva encompasses the external structures of the female reproductive system. At the forefront is the monpubis, a cushion of fatty tissue atop the pubic bone. Once puberty sets in, this area typically grows hair. Extending from just behind the mons pubis are the labia majora (labia = 'lips'; majora = 'larger'), which are larger skin fs olds coated with hair. Nestled within are the labia minora (labia = 'lips'; minora = 'smaller'), which are thinner, more pigmented, and hairless. While the...
General External Flow Characteristics01:26

General External Flow Characteristics

The study of external flow is essential for creating structures and objects that interact efficiently and safely with moving fluids, such as air or water. When a body is immersed in a flowing fluid, it experiences two primary forces: drag, which opposes motion along the flow direction, and lift, which acts perpendicular to the flow. The shape, size, and orientation of the object influence these forces.Streamlined and Blunt Bodies in External FlowObjects in fluid flow are classified as...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Adherence to international pharmacogenomic recommendations in pediatric cancer care: a cohort analysis embedded within the MARVEL-PIC randomized trial.

Pharmacogenomics·2026
Same author

Molecular basis of polyadenylated RNA fate determination in the nucleus.

Nature·2026
Same author

Tacrolimus in Pediatric Solid Organ Transplant Recipients: A Retrospective Cohort Study.

Pediatric transplantation·2026
Same author

Mechanism of RACK1-dependent ZAKα activation at stalled and collided ribosomes.

Molecular cell·2026
Same author

Clinical Pharmacogenetics Implementation Consortium (CPIC) Guideline for CYP2D6 Genotype and Use of 5-HT<sub>3</sub> Receptor Antagonists: 2026 Update.

Clinical pharmacology and therapeutics·2026
Same author

Real-World Applications of Pharmacogenomics in Paediatrics.

Journal of paediatrics and child health·2025

相关实验视频

Updated: Jul 8, 2026

Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement
09:50

Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement

Published on: October 1, 2014

重生了外体的外体.

Torben Heick Jensen1, Claire Moore

  • 1Department of Molecular Biology, Arhus University, 8000 Arhus C., Denmark.

Cell
|June 7, 2005
PubMed
概括

外基因组复合体对于真核细胞中的RNA衰变至关重要,其体外活性较弱. 新的研究揭示了一种RNA衰变激活复合物与多聚A) 聚合酶,解释了这种活性,并确定了新的转录单元.

科学领域:

  • 分子生物学分子生物学
  • 基因规则 基因规则
  • 在RNA代谢过程中.

背景情况:

  • 外体复合体是真核核中3'-5'RNA衰变的主要机制.
  • 纯化的外体在体外表现出有限的催化活性,对其生物功能构成了难题.

研究的目的:

  • 研究调节外体介导RNA衰变的因素.
  • 在体外 (in vitro) 识别增强外体细胞活动的成分.
  • 为了描述新型RNA聚合酶II转录单元.

主要方法:

  • 生物化学测试以评估外体活动.
  • 蛋白质复合物的净化和表征.
  • 对RNA聚合酶II转录的分析.

主要成果:

  • 发现了一种含有多A聚合酶的新型RNA衰变激活复合物.
  • 这种复合物显著增强了外体的体外活性.
  • 发现了新的非传统的RNA聚合酶II转录单元.

结论:

  • 新发现的复合物对于有效的核RNA衰变至关重要.

更多相关视频

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
08:49

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii
03:37

Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii

Published on: May 6, 2022

相关实验视频

Last Updated: Jul 8, 2026

Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement
09:50

Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement

Published on: October 1, 2014

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
08:49

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii
03:37

Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii

Published on: May 6, 2022

  • 这一发现解决了纯化外体的低体外活性悖论.
  • 这些发现揭示了涉及转录和衰变的复杂基因调节机制.