对于依赖RNAi的异染色组件,需要RNA聚合酶II
Hiroaki Kato1, Derek B Goto, Robert A Martienssen
1Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
概括
在RNA聚合酶II (RNAPII) 中的一种突变破坏了裂变酵母中的异染色素组合. 这一发现揭示了RNAPIIII.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 在Schizosaccharomyces pombe中,RNA干扰 (RNAi) 途径对于产生小干扰RNA (siRNA) 是至关重要的.
- siRNAs对于形成危中心性异色染色体,一种沉默的染色体状态至关重要.
- RNA聚合酶II (RNAPII) 在整个基因组中转录基因,包括周心区域.
研究的目的:
- 为了研究RNA聚合酶II (RNAPII) 在周围中心的异色素蛋白形成中的作用.
- 描述特定RNAPII突变对RNA干扰 (RNAi) 和异染色蛋白的影响.
主要方法:
- 对一种新的Schizosaccharomyces pombe突变的基因分析,该突变在RNAPII的第二大子单元中存在缺陷.
- 显微镜评估野生类型和突变RNAPII的局部化到周心质.
- 对异色色素基因组修饰的分析.
- 对周心层转录和小干扰RNAs (siRNAs) 的量化.
主要成果:
- 在RNAPII的突变并没有影响其定位到周心层.
- 突变细胞表现出异色色素基因组修饰的丧失.
- 在突变细胞中积累的围心转录.
- 在突变者中,siRNA水平显著降低.
结论:
- RNAPII在将周心转录与siRNA处理相结合方面发挥着直接作用.
- 研究的RNAPII突变通过影响RNAi路径组件,损害了异染色蛋白组合.
- 这些发现突出了RNAPII在通过表观遗传调节来维持基因组稳定的新功能.
相关概念视频
RNA Polymerase II Accessory Proteins
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Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...


