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寡核酸探针与自组装单层的化学选择性共价合

Neal K Devaraj¹, Gregory P Miller, Wataru Ebina

¹Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.

Journal of the American Chemical Society

|June 16, 2005

概括

一种新的方法使用铜催化点击化学快速将寡核酸探针连接到表面. 这种化学选择性路径提供了对探针密度和功能的控制,尽管存在杂质,简化了表面功能.

科学领域:

化学生物学化学生物学
材料科学材料科学材料科学
表面化学表面化学

背景情况:

将寡核酸探针连接到表面对于各种应用至关重要,包括生物传感器和诊断.
传统的方法通常涉及核性-电性合,这可能是缓慢的,对杂质敏感.
开发强大而高效的表面功能化技术是一个持续的挑战.

研究的目的:

介绍一种常规和快速的化学选择方法,用于将寡核酸探针连接到明确的表面.
为了展示一种使用铜 (I) 催化亚酸循环添加 (CuAAC) 的新方法,点击化学.
为了控制表面的寡核酸探针密度.

主要方法:

使用的Cu ((I) tris ((benzyltriazolylmethyl) 胺催化合终端乙烯与在自组装单层上存在的化物.
研究了在存在核友和电友杂质的情况下反应的效率.
通过调整表面上的azide功能来证明对探头密度的控制.

主要成果:

成功地将寡核酸探针与高效率和速度连接到黄金表面.
CuAAC的点击化学反应被证明是强大的,耐受各种杂质.
通过调节表面亚化物度,有效控制了寡核酸探针密度.
该方法在用azidotrimethoxysilane修改的玻璃幻灯片上进行了初步验证.

结论:

提出的化学选择性路径提供了一种常规和快速的方法,用于将寡核酸探头附着在表面上.
铜催化点击化学为传统合反应提供了强大的替代方案,在存在杂质的情况下表现出色.
这种技术允许控制表面功能化,并且广泛适用于各种表面,适用于含有亚的单层组装.

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