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Updated: Aug 14, 2026

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
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Published on: May 29, 2011

通过光探测到未溶解的Trp蛋白的形状变化

Anthony T Iavarone1, Joel H Parks

  • 1The Rowland Institute at Harvard, 100 Edwin H. Land Boulevard, Cambridge, Massachusetts 02142, USA.

Journal of the American Chemical Society
|June 16, 2005
PubMed
概括

研究人员直接测量了Trp-cage在气相中的蛋白质展开. 和的变化大于溶液中的变化,并且根据电荷状态而异.

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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...