双立方体ISES:在现场估计催化剂选中的反选择性和相对速率
Sangeeta Dey1, Kannan R Karukurichi, Weijun Shen
1Department of Chemistry, University of Nebraska, Lincoln, Nebraska 68588-0304, USA.
Journal of the American Chemical Society
|June 16, 2005
概括
一种新的现场酶选 (ISES) 方法有效地选催化剂的酶选择性和反应速率. 这种双立方体方法快速识别出用于水解动力学分辨率的最佳性性催化剂.
科学领域:
- 不对称的催化剂.
- 酵素工程是什么意思 酵素工程
- 高通量选 高通量选
背景情况:
- 在非对称合成中,选合催化剂以检测其酶选择性和反应速率至关重要.
- 现有的方法可能耗时,需要大量的催化剂和基质.
- 现场监测为催化剂评估提供了一种更有效的方法.
研究的目的:
- 引入和验证一种新型的双体现场酶查 (ISES) 方法.
- 为了评估一个聚焦的性性催化剂库的enantioselectivity和相对率.
- 为了证明ISES在识别有前途的催化剂组合方面的实用性.
主要方法:
- 开发了一种具有明显有机和水分层的双 ISES 系统.
- 作为一个模型反应,利用了氧化的Co (III) -salen催化液解动力学分辨率 (HKR).
- 在水层中使用报告酶来监测二醇形成和反体比率 (R:S).
主要成果:
- 成功证明了双子ISES方法的原理证明.
- 选了一套性性催化剂库,确定了几种有效组合.
- 观察到甲伙伴对催化剂反选择的显著影响.
- 与独立测量值相关的ISES预测的enantioselectivities (ee's) 和相对率.
结论:
- 双 ISES 方法提供了一个快速高效的平台,用于选性催化剂.
- 这种方法有助于发现具有量身定制的酶选择活性的新型催化剂结构.
- ISES是优化不对称反应和催化剂开发的宝贵工具.
相关概念视频
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