在S. cerevisiae中基因组位置的基因组规模识别
Guo-Cheng Yuan1, Yuen-Jong Liu, Michael F Dion
1Bauer Center for Genomics Research, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA.
Science (New York, N.Y.)
|June 18, 2005
概括
核细胞的定位通过控制DNA可访问性来调节基因表达. 这项研究绘制了酵母中的核细胞组图,揭示了转录因子访问的促销者处保存的,组织良好的染色质结构.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 核细胞的定位对于真核生物的基因表达调节至关重要.
- 将DNA包装到核体中会影响序列的可访问性.
- 了解染色体组织是解读基因调节的关键.
研究的目的:
- 在Saccharomyces cerevisiae基因组的很大一部分中,以高分辨率绘制核位.
- 调查核细胞定位与促进体等调控元素之间的关系.
- 确定核细胞定位在控制转录因子结合中的作用.
主要方法:
- 开发一种高分辨率的型微阵列方法.
- 核细胞转化位置的全基因组映射.
- 在Saccharomyces cerevisiae中分析染色体组织,重点是染色体III和调节区域.
主要成果:
- 在酵母DNA的482千基基上鉴定出2278个位置良好的核细胞.
- 在Pol II促进体中发现了一种保存的染色质结构:一个无核细胞体的区域在起始编码子上游,周围是定位的核细胞体.
- 观察到无核细胞体区域在进化过程中得到保护,并富含多dA/dT序列.
- 发现转录因子结合基因在很大程度上是无核细胞体的,这表明核细胞体的定位是访问的决定因素.
结论:
- 核细胞的定位是调节基因表达的一个主要因素.
- 在酵母促进体中存在一种刻板的染色质组织,影响转录因子的可访问性.
- 核细胞的定位在确定转录因子对DNA基因的访问方面发挥着全球作用.
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