Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Cystobactamid off-target profiling reveals favorable safety, superoxide reduction, and SCARB1 inhibition in eukaryotes.

npj drug discovery·2026
Same author

Centrifugal microfluidic automation of the protein aggregation capture workflow for robust mass spectrometry-based proteomics.

Lab on a chip·2026
Same author

Natural Product-Derived Ianthelliformisamines Inhibit Protein Translation and Block Bacterial Flagellum Assembly.

ACS chemical biology·2026
Same author

Bioresponsive pseudoGlucosinolates (psGSLs) Release Isothiocyanates (ITCs) in the Presence of Nitroreductases.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Chemical Proteomics Reveal the Inventory of Pyrroloquinoline Quinone Binding Proteins in Bacteria.

Journal of the American Chemical Society·2026
Same author

Metronidazole and ether derivatives target Helicobacter pylori via simultaneous stress induction and inhibition.

Nature microbiology·2026

相关实验视频

Updated: Jun 25, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

化学酶方法对B变异的抗纯链路蛋白:从Streptomyces pristinaespiralis中对立体选择性普里斯胺素I环酶的表征.

Christoph Mahlert1, Stephan A Sieber, Jan Grünewald

  • 1Fachbereich Chemie/Biochemie, Philipps-Universität Marburg, Hans-Meerwein-Strasse, D-35032 Marburg, Germany.

Journal of the American Chemical Society
|June 30, 2005
PubMed
概括

我们开发了一种新型的化学酶策略,用于合成链条蛋白B型抗生素. 这种方法使用酶性循环处理来克服制造这些最后一线防御抗微生物药物的挑战.

更多相关视频

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
10:43

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Published on: October 3, 2019

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

相关实验视频

Last Updated: Jun 25, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
10:43

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Published on: October 3, 2019

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

科学领域:

  • * 生物化学和合成化学
  • * * 自然产品合成
  • * 发现一种抗菌药物.

背景情况:

  • * 链条蛋白B型抗生素是对抗耐药细菌的最后一线防御药物.
  • * 这些循环的化学合成是具有挑战性的,因为种族化敏感性.
  • *需要新的策略,以有效地获得多种类型的Streptogramin B类似物.

研究的目的:

  • * 开发一种化学酶策略,用于快速多样化合成B型抗生素.
  • *以其循环化能力来表征 thioesterase 域 SnbDE TE.
  • * 为了使新型的Streptogramin B变体能够产生新的Streptogramin B变体,这些变体具有改善活动的潜力.

主要方法:

  • *从Streptomyces pristinaespiralis. 的SnbDE TE域的克隆,过度生产和生物化学特征.
  • *利用固相合成来生成骨和酶循环来实现立体选择性.
  • * 采用动态动力学分辨率试验用于种族化基质的立体选择性循环.

主要成果:

  • * SnbDE TE可催化基的区域选择性和立体选择性循环,包括复杂混合物.
  • * 该酶在骨中表现出广泛的基质耐受性.
  • * SnbDE TE是第一个能够同时进行巨乳化和巨乳形成的已识别的环酶.
  • *在4位和5位之间的N-甲基化键对于高酶周转是必不可少的.

结论:

  • * 化学酶策略提供了有效的获取多种Streptogramin B类似物.
  • * SnbDE TE是一种用于合成循环抗生素的多功能酶.
  • *这种方法有助于改善抗菌剂的结构-活性关系研究.