在实体瘤中发现癌症基因,在小鼠中使用基于转子体的体质突变发生
Lara S Collier1, Corey M Carlson, Shruthi Ravimohan
1Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, The Cancer Center, The University of Minnesota Twin Cities, Minneapolis, Minnesota 55455, USA.
睡眠美女转子可以识别各种小鼠组织中的癌症基因. 这种工具使得无偏见的,前的基因查促进瘤形成和癌症基因的发现 in vivo.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 逆转录病毒是确立的体细胞插入性变异原体,用于在特定组织中识别癌症基因.
- 存在对插入性变异原体的需求,适用于更广泛的体细胞和组织,以发现癌症基因.
研究的目的:
- 评估睡眠美女转子作为体质插入性突变原体,用于识别参与固体瘤形成的基因.
- 为了证明其实用性,在无偏见的,转发癌症基因的基因选 in vivo.
主要方法:
- 为功能丧失或功能获取突变设计一个睡眠美女转位子.
- 在小鼠体内各种体质组织中评估转子子转位.
- 在易患癌症的小鼠中诱导和分析瘤形成.
- 从瘤中克隆和分析转子子插入部位.
主要成果:
- 睡眠美女转体子在所有测试的体质组织中成功转体.
- 它在易患癌症的小鼠中加速了瘤的形成.
- 在已知和候选癌症基因中确定了常见的整合部位,反映了逆转录病毒突变发生的发现.
结论:
- 睡眠美女转位子是一种有效的体质插入突变原体,用于识别参与固体瘤形成的基因.
- 它代表了一种新的工具,用于无偏见的,体内先进的基因查,以在各种组织中发现癌症基因.
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