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一种类似Arabidopsis hAT的转化酶对植物发育至关重要
1Institute for Biology, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands.
Nature
|July 15, 2005
概括
类似转移酶的基因,如Arabidopsis中的DAYSLEEPER,对于植物发育和基因调节至关重要. 这些化的转基因酶对于正常生长至关重要,并影响全球基因表达模式.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 植物生物学 植物生物学
背景情况:
- 可转移的元素占植物和脊椎动物基因组的很大一部分.
- 自主DNA转位子使用转位酶进行"切割粘贴"动员,这种动员具有突变性.
- 宿主生物利用表观遗传沉默和异染色体的形成来限制转移.
研究的目的:
- 为了研究来自Arabidopsis thaliana的新型转体酶的功能,命名为DAYSLEEPER.
- 确定DAYSLEEPER在植物生长和基因表达中的作用.
- 为了探索转移酶对细胞功能的潜在化.
主要方法:
- 基于DAYSLEEPER与ArabidopsisDNA修复基因Ku70.0.的促进子区域中的Kubox1动机的结合而分离的DAYSLEEPER.
- 在DAYSLEEPER淘汰和过度表达线路中分析植物生长和发育.
- 全球基因表达在植物中变化的DAYSLEEPER水平的分析.
主要成果:
- 日常睡眠酶 (DAYSLEEPER) 是一种与hAT家族相关的转移酶,对于Arabidopsis thaliana正常发育至关重要.
- 缺乏DAYSLEEPER或过度表达它的植物表现出异常生长表型.
- 过度表达导致许多基因表达的显著改变.
- 由DAYSLEEPER识别的Kubox1动机在许多植物基因的上游发现.
结论:
- 类似转移酶的基因可以被化,并成为植物发育的必需品.
- 日日在调节植物的全球基因表达方面发挥着至关重要的作用.
- 化转化酶可以完成重要的细胞功能,超出它们在转化中的祖先作用.
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