相关实验视频

Updated: Jul 18, 2026

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
04:37

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

Published on: June 29, 2021

在液体乙中封装蛋白质的高分辨率NMR研究

Ronald W Peterson1, Brian G Lefebvre, A Joshua Wand

  • 1Johnson Research Foundation and Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6059, USA.

Journal of the American Chemical Society
|July 21, 2005
PubMed
概括

研究人员使用液态乙将蛋白质封装在反向微粒中,增强分子重定向以获得更好的溶液NMR光谱. 这种方法改善了用于研究大型蛋白质的旋转旋转NMR放松时间.

更多相关视频

Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue07:40

Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue

Published on: May 17, 2024

Exploring Protein-Glycan Interactions: Advances in Nuclear Magnetic Resonance10:07

Exploring Protein-Glycan Interactions: Advances in Nuclear Magnetic Resonance

Published on: August 26, 2025

相关概念视频

¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...