超对磁性脂质体的生成被证明是用于体内成像的高效MRI对比剂
Marie-Sophie Martina1, Jean-Paul Fortin, Christine Ménager
1Laboratoire de Physico-Chimie des Systèmes Polyphasés, UMR CNRS 8612, Université Paris-Sud, Faculté de Pharmacie, 5 rue Jean-Baptiste Clément, F-92296 Châtenay-Malabry Cedex, France.
Journal of the American Chemical Society
|July 28, 2005
概括
合成磁性液体载荷脂质体 (MFLs) 使用maghemite纳米晶体和PEG-化脂质. 这些MFL显示出出色的稳定性,MRI的T2对比度增强,以及体内隐形性质,使磁导成为可能.
科学领域:
- 纳米技术和材料科学 材料科学
- 生物医学工程 生物医学工程
- 放射学和医学成像学 医学成像学
背景情况:
- 开发稳定和有效的磁性纳米粒子用于生物医学应用是至关重要的.
- 脂质体封装提供了一种稳定纳米粒子并提高其生物相容性的方法.
- 聚乙烯糖醇 (PEG) 化对于提高循环时间和减少纳米颗粒的非特异性吸收至关重要.
研究的目的:
- 为了合成和表征maghemite (gamma-Fe2O3) 纳米晶体载荷脂质体 (MFLs).
- 评估MFLs的物理化学特性,稳定性和MRI对比增强能力.
- 评估PEG-ylated MFLs用于磁共振成像 (MRI) 的体内性能和隐形性质.
主要方法:
- 通过薄膜水化和挤出,合成maghemite纳米晶体,并将其封装在大型单囊囊中 (卵酸胆和DSPE-PEG2000).
- 使用闪光凝排除色谱进行净化.
- 使用准弹性光散射,低温传输电子显微镜,磁化曲线记录和MR缓解度测量进行了表征.
- 在试验室对J774巨细胞系和小鼠体内MRI的毒性测定.
主要成果:
- 均的MFLs (195 +/- 33nm) 与可调节的铁负载 (35-167 Fe(III) /脂质mol %) 已成功准备好.
- 封装保存了maghemite纳米晶体的物理和超偏磁性质.
- MFLs在生物介质中表现出高稳定性,无毒性,由于PEGylation而减少的巨细胞关联,以及优异的T2对比增强 (r2/r1比为6-18).
- 在体内MRI显示了有效的对比度增强,并证实了PEG-ylated MFLs的隐蔽行为.
结论:
- 带有PEG化磁性流体载荷的脂质体是MRI的稳定,无毒和高效的T2对比剂.
- 由PEGylation赋予的隐形性质显著降低了巨细胞对脂质细胞的吸收.
- 这些MFL有望用于先进的MRI应用,包括MRI血管造影,并有可能用于磁场引导的输送.
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