果核受体e75含有血红素,并且对气体有反应
Jeff Reinking1, Mandy M S Lam, Keith Pardee
1Banting and Best Department of Medical Research, Charles H. Best Institute, 112 College Street, Toronto, Ontario, M5G 1L6, Canada.
Cell
|July 30, 2005
概括
果核受体E75含有海姆组,作为海姆水平,氧化还原状态和NO和CO等二原子气体的传感器. 这一发现揭示了核受体的新型调节机制.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 核受体是调节基因表达的转录因子.
- 许多核受体仍然是"孤儿受体",因为未识别的配体.
- 果虫E75核受体的功能尚未完全理解.
研究的目的:
- 为了研究Drosophila核受体E75.5的连接体结合域.
- 为了识别E75联体结合口袋中的潜在联体或假体组.
- 探索任何已识别的分子对E75活动的功能影响.
主要方法:
- 对E75吸收光谱的分析.
- 评估E75对变质剂的耐药性.
- E75蛋白的局部导向突变发生.
主要成果:
- 在E75连接体结合口袋内显示出一种血红蛋白假体组的存在.
- 光谱,变性耐性和突变发生证实了一个单一的,协调结合的heme.
- E75表达与可用的血红素相关,表明血红素感应作用.
- 黑米铁的氧化状态调节了E75与DHR3的相互作用,这表明有氧还原感应.
- 结合或CO的调节了E75-DHR3的相互作用,这表明二原子气体的传感.
结论:
- 草核受体E75具有一个血红蛋白假体组.
- E75作为,氧化还原状态和二原子气体 (NO,CO) 的多传感器.
- 这一发现揭示了涉及血的核受体的新型调节机制.
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