瘟疫细菌在感染期间向免疫细胞
Melanie M Marketon1, R William DePaolo, Kristin L DeBord
1Department of Microbiology, University of Chicago, Chicago, IL 60637, USA.
概括
瘟疫细菌 (Yersinia pestis) 在感染期间注射免疫细胞,主要是树突细胞,巨细胞和中性粒细胞. 这种细菌策略可能会使宿主免疫反应失效,导致瘟疫.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
背景情况:
- 瘟疫是一种严重的传染病,由Yersinia pestis引起.
- 耶尔西尼亚瘟疫利用一种III型分泌系统,将效应蛋白 (Yops) 注入宿主细胞中.
- 感染期间Yersinia pestis的特定宿主细胞点在很大程度上仍未确定.
研究的目的:
- 为了识别特定的宿主免疫细胞群体,这些细胞群体是Yersinia pestis的点,用于效应蛋白注射.
- 了解Yersinia pestis在瘟疫期间操纵宿主免疫反应的机制.
主要方法:
- 使用Yopβ-乳糖酶混合蛋白来跟踪效应器注射.
- 在从瘟疫感染的动物中分离出来的活细胞上采用光染色技术.
- 分析了各种免疫细胞类型的注射频率,包括树突细胞,巨细胞,中性粒细胞,B淋巴细胞和T淋巴细胞.
主要成果:
- 耶尔西尼亚瘟疫优先选择免疫细胞用于Yop蛋白注射.
- 状细胞,巨细胞和中性粒细胞是体内最常被注射的细胞类型.
- B淋巴细胞和T淋巴细胞很少成为Yersinia pestis注射的目标.
结论:
- 耶尔西尼亚瘟疫积极向关键的免疫细胞群体,以破坏.
- 将效应蛋白注入免疫细胞,很可能会使宿主免疫系统失效.
- 这种有针对性的免疫细胞攻击是导致瘟疫病变的关键机制.
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